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| 基于荧光素酶发光体系测试饮用水中农药的综合毒性 |
| Determ ination of Synthetic Toxicity of Pesticidesin Drinking Water Based on Inhibition of Biolum inescence from Luciferase Reacting System |
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| DOI： |
| 中文关键词: 荧光素酶 发光体系 农药 综合毒性 饮用水 |
| 英文关键词: Luciferase Luminescence system Pesticides Synthetictoxicity Drinking water |
| 基金项目:国家科技支撑计划世博科技专项基金资助项目 (06dz05825) |
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| 中文摘要: |
| 利用农药对荧光素酶催化的发光反应具有非常显著的抑制作用, 对甲拌磷、乐果、毒死蜱、百草枯等4种农药分别进行单一毒性和等比混合法联合毒性测试, 建立了一种快速检测饮用水中农药综合毒性的生物学方法。试验结果表明, 单一农药乐果、甲拌磷、百草枯和毒死蜱的 EC50 值分别为 7.56 mg/L 、12.7 mg/L 、19.0 mg/L 和 65.3 mg/L, 毒性强弱顺序为乐果 > 甲拌磷 > 百草枯 > 毒死蜱 , 相关系数 ≥ 0.995; 将 4 种农药以等比方式配制成两两混合液后, 当质量浓度为20 |
| 英文摘要: |
| A fastdetermination method wasestablished forsynthetictoxicityin drinking water. Fourpesti cides(thimet, cygon,chlorpyrifosand paraquat) were usedtoinvestigatesingletoxicity and synthetictoxicity by classic method ofequaldose 2 effectexperimentwithinhibition ofbioluminescencefrom luciferasereactingsystem. Resultsshowed that EC 50 were thimet7.56 mg/L, cygon 12.7 mg/L, chlorpyrifos19.0 mg/L and paraquat65.3 mg/L respectively. Toxicitylevelorderofthe pesticides wasfollowing: cygon >thimet>paraquat> chlor pyrifos. The related coefficient was ≥ 0.995. When any two ofthe pesticides from range of20.0 mg/L to 100 mg/L were mixed based on equaltoxicity ofsingle pesticide, antagonistic effectwas manifested exceptthat mixtureofparaquatand chlorpyrifosshowed the synergetic effect. |
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