| A method was established for determination of four arsenic forms such as As (Ⅲ), methanearsenic acid (MMAV), dimethyl arsenic acid (DMAV) and As(Ⅴ) in soil samples by high performance liquid chromatography (HPLC) hydride generation (HG) atomic fluorescence spectrophotometry (AFS). Phosphoric acid was used as extractive solution, Ascorbic acid as reducing agent. In optimized conditions, samples were bathed in water for extracting different arsenic forms before the determination. The four arsenic forms were separated in 7 min. The good linearity of four arsenic forms was obtained in range from 1. 00 μg/L to 100 μg/L, the laboratory detection limits for As (Ⅲ) 0.25 μg/L, DMAV 0.36 μg/L, MMAV 0.39 μg/L and As (Ⅴ) 0.51 μg/L respectively, relative standard deviation of duplicates of soil reference materials≤7.4%, spiked recoveries between 79.5% and 95.0%, extractive rate from 74.6% to 90.4%. |
