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| PCR技术在水生毒理学研究中的应用 |
| Application of PCR Technology for Aquatic Toxicologic Research |
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| DOI： |
| 中文关键词: |
| 英文关键词: |
| 基金项目:国家自然科学基金资助项目（50830304） |
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| 中文摘要: |
| 介绍了聚合酶链式反应（PCR）技术的基本原理，并在此基础上综述了PCR技术在水生毒理学研究中的应用及国内外最新研究进展，其中涉及的模式生物包括原生动物、浮游植物、浮游动物和鱼类等。PCR技术不仅为污染物对水生生物的毒性检测带来了便利，还有助于从分子水平上阐述污染物的毒性作用机制。最后指出了目前PCR技术应用的局限性及未来的发展前景。 |
| 英文摘要: |
| Technologic principle of polymer chain reaction (PCR) was described. Research development of PCR technology for the application of aquatic toxicology was reviewed, which model organism included protozoa, zooplankton, phytoplankton and fishes at home and abroad. PCR technology not only brought convenient for toxic detection of pollutant by using aquatic organisms but also help for explanation of toxic pollutants mechanism at molecular level. Limitation and development of PCR in the future application were also discussed. |
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