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| 快速检测痕量铜的光电型传感器的研制 |
| Research of Photoelectric Sensor for Fast Test of Trace Copper |
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| 中文关键词: |
| 英文关键词: |
| 基金项目:上海市科委技术标准专项基金资助项目（06DZ05036） |
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| 中文摘要: |
| 利用铜对葡萄糖氧化酶的抑制作用，以及葡萄糖氧化酶-辣根过氧化物酶-葡萄糖（Glu）-邻联甲苯胺（OT）偶联反应体系生成蓝色底物的原理，将Glu与OT固定化制成试纸条，插入自制的便携式单色光反射计，组装出检测铜的光电型传感器。当铜的质量浓度范围为1 mg/L~100 mg/L时，光反射强度值与铜的质量浓度呈良好的线性关系。在优化的试验条件下，该方法的检测限为0.064 mg/L，检测时间为5 min。 |
| 英文摘要: |
| A photoelectric sensor was composed by home-made portable monochromatic light reflector which inserted a test strip with immobilized glucose and o-tolidine for copper detection. Principle of the photoelectric sensor was that inhibitory effect of copper on glucose oxidase formed blue substrate by using a coupling reaction system of glucose oxidase-horseradish peroxidase-glucose-o-tolidine. There was good correlative linearity between 1 mg/L~100 mg/L copper solution and light reflecting intensity. The detection limit of method was 0.064 mg/L, testing time 5 minutes after optimization of the experimental condition. |
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