| Using chloramphenicol (CAP) as the template, methyl acrylamide as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the cross linker, methanol as the solvent, ionic liquid (［BMIm］BF4) as the porogenic solvents, azodiisobutyronitrile (AIBN) as the initiator, a chloramphenicol imprinted monolithic column was prepared by in situ molecular imprinting technique. The synthesis conditions were optimized, and the selectivity of chloramphenicol imprinted monolithic column was investigated. The imprinting effect of CAP molecularly imprinted monolithic columns prepared by ionic liquids was compared with traditional polymer. The method was developed for analysis of the extraction of milk spiked with chloramphenicol. The result showed that the impurities and target molecule could be separated even under the complex matrix environment. |
