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| 水体中藻类叶绿素a提取方法的比较 |
| Comparison for Extracting Chlorophyll a from Algae in Water |
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| DOI： |
| 中文关键词: 叶绿素a 藻类 提取方法 水质 |
| 英文关键词: Chlorophyll a Algae Extraction method Water quality |
| 基金项目:江苏省环保科研基金资助项目（2015035） |
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| 中文摘要: |
| 考察超声波法、反复冻融法、热乙醇法对小球藻和微囊藻中叶绿素a的提取效果，并与标准方法作比对。结果表明，4种提取方法中，标准方法对藻类提取效果稍逊，热乙醇法对小球藻的提取效果好，而超声波法和反复冻融法对微囊藻的提取效果好。建议针对不同水体中的优势藻类，标准方法可结合热乙醇法和超声波法，用乙醇替换丙酮作为叶绿素a的提取溶剂，并增加超声波破碎步骤，以提高叶绿素a的提取效率。 |
| 英文摘要: |
| In order to study the extraction effect of chlorophyll a from chlorella and microcystis, the ultrasonic method, repeated freezing and thawing method and hot ethanol method were chosen to compare with the standard method. The results showed that the extraction effect of standard method on algae was poor, but on chlorella the extraction effect of hot ethanol method was better, while the extraction effect of ultrasonic method and repeated freezing and thawing method on microcystis were both better. It suggested replacing acetone with ethanol as the solvent for chlorophyll a extraction and increasing the ultrasonic crushing step, so as to improve the extraction efficiency of chlorophyll a. |
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