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| LC-MS/MS法测定沉积物中磺胺类和四环素类抗生素 |
| Simultaneous Determination of Sulfonamide and Tetracycline Antibiotics Residues in Sediment using LC MS/MS |
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| DOI： |
| 中文关键词: 磺胺类抗生素 四环素类抗生素 液相色谱-串联质谱法 沉积物 |
| 英文关键词: Sulfonamide antibiotics Tetracycline antibiotics LC MS/MS Sediment |
| 基金项目:福建省环保科技计划基金资助项目（2016R004） |
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| 摘要点击次数: 724 |
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| 中文摘要: |
| 沉积物样品用乙腈-柠檬酸缓冲溶液提取后经HLB-SPE柱净化，采用LC-MS/MS法在MRM模式下测定17种磺胺类和4种四环素类抗生素，通过优化测定条件，使方法在0.500 μg/kg~100 μg/kg范围内线性良好。方法检出限为0.15 μg/kg~1.2 μg/kg，2个质量比水平的加标回收率为44.1%~125%，试验5次结果的RSD<16.0%。将该方法用于测定厦门近岸海域沉积物中抗生素，共有6种磺胺类和4种四环素类抗生素检出，测定值为0.3 μg/kg~26.2 μg/kg。 |
| 英文摘要: |
| Sediment samples were extracted by acetonitrile citric acid buffer solution and purified by HLB SPE column. Seventeen sulfonamides and four tetracyclines were determined by liquid chromatography tandem mass spectrometry under multi reaction monitoring mode(MRM). By optimizing the determination conditions, the linearity of the method was good in the range of 0.500 μg/kg~100 μg/kg. The detection limits were 0.15 μg/kg~1.2 μg/kg. The recoveries of standard addition were in range of 44.1%~125% at two mass ratio levels. The RSD of 5 duplicate measurements was less than 16.0%. The method was applied to determine antibiotics in sediments from Xiamen coastal area in Fujian Province. Six sulfonamides and four tetracyclines were detected and the content ranged in 0.3 μg/kg~26.2 μg/kg. |
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