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| UPLC-MS/MS法测定尿液中的反-反黏糠酸和苯巯基尿酸 |
| Determination of Trans，Trans Muconic Acid and S Phenylmercaputuric Acid in Urine Samples by UPLC MS/MS Method |
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| DOI： |
| 中文关键词: 反-反黏糠酸 苯巯基尿酸 超高效液相色谱-串联质谱法 尿液 |
| 英文关键词: Trans,trans muconic acid S phenylmercaputuric acid UPLC MS/MS Urine |
| 基金项目:江苏省医学重点学科-流行病学基金资助项目(ZDXKA2016008)；江苏省青年医学人才基金资助项目(QNRC2016543) |
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| 中文摘要: |
| 采用超高效液相色谱-串联质谱法(UPLC-MS/MS)测定尿液中的苯代谢产物反-反黏糠酸与苯巯基尿酸，通过优化前处理和测定条件，使反-反黏糠酸在100 μg/L～1 000 μg/L范围内，苯巯基尿酸在0500 μg/L～500 μg/L范围内线性良好，方法检出限为14 μg/L〖JP3〗和008 μg/L。将该方法用于实际尿样的加标回收试验，结果加标回收率为906%～〖JP〗108%，6次测定结果的RSD为15%～42%，有证标准物质测定结果在规定范围内。 |
| 英文摘要: |
| A method for determining benzene metabolite， trans, trans muconic acid and S phenylmercaputuric acid, in urine by ultra high performance liquid chromatography tandem with mass spectrometry was established. By optimizing the sample pretreatment and determination conditions, the method had good linearity in the range of 10.0 μg/L~1 000 μg/L for trans, trans muconic acid and 0.500 μg/L~50.0 μg/L for S phenylmercaputuric acid. The detection limits oftrans， trans muconic acid and S phenylmercaputuric acid were 1.4 μg/L and 0.08 μg/L respectively. The recoveries of the target 〖JP3〗compounds in real samples were in the range of 90.6%~〖JP〗108%. The RSDs were in the range of 1.5%~4.2%(n=6). The determination results of certified reference materials were within the prescribed range. |
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