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| HPLC-高分辨飞行时间质谱测定水中多种微囊藻毒素 |
| Determination of Microcystins in Water by HPLC Quadrupole High Resolution Time of Flight Mass Spectrometry |
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| DOI： |
| 中文关键词: 微囊藻毒素 固相萃取 高效液相色谱法 高分辨飞行时间质谱法 水质 |
| 英文关键词: Microcystin Solid phase extraction High performance liquid chromatography High resolution time of flight mass spectrometry Water quality |
| 基金项目:拱北海关科技计划基金资助项目（ZH2017-36） |
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| 中文摘要: |
| 水样经HLB小柱富集、净化后，采用高效液相色谱-四极杆-高分辨飞行时间质谱同时测定水样中MC-LA、MC-LF、〖JP3〗MC-LR、MC-LW、MC-LY、MC-RR、MC-YR、MC-WR等8种微囊藻毒素。试验表明，8种微囊藻毒素在0500 μg/L～〖JP〗100 μg/L范围内线性良好，方法检出限为0001 μg/L～0005 μg/L，自来水空白样品3个质量浓度水平的加标回收率为725%～998%，5次测定结果的RSD为19%～112%。将该方法用于测定珠海本地水产养殖鱼塘和小型水库的水样，结果均未检出 |
| 英文摘要: |
| 8 kinds of microcystin， MC LA， MC LF， MC LR， MC LW， MC LY， MC RR， MC YR and MC WR， in water were purified and enriched by HLB column and determined simultaneously by high performance liquid chromatography quadrupole high resolution time of flight mass spectrometry (HPLC Q TOF). The experimental results showed that the calibration curves of microcystins had good linearity in the range of 0.500 μg/L to 100 μg/L. The detection limits were between 0.001 μg/L and 0.005 μg/L. The recoveries of blank samples at three mass concentration levels were from 72.5% to 99.8%， the RSDs of five measurements were from 1.9% to 11.2%. The method was applied in monitoring the water samples from local aquaculture fishponds and small reservoirs in Zhuhai， the results of the 8 kinds of microcystin were not detected. |
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