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| 飞行时间质谱-GC-MS/MS法测定桂花中β-紫罗兰酮 |
| Determination of β-Ionone in Osmanthus fragrans (Thunb.)Lour. by GC-Q-TOF MS and GC-MS/MS |
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| DOI： |
| 中文关键词: β-紫罗兰酮 飞行时间质谱法 气相色谱三重四级杆串联质谱法 桂花 |
| 英文关键词: β-ionone GC-Q-TOF/MS GC-MS/MS Osmanthus fragrans (Thunb.) Lour. |
| 基金项目:国家科技重大专项子课题“太湖流域浮游生物快速鉴定技术验证及应用转化”基金资助项目(2018ZX07208002-02) |
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| 中文摘要: |
| 采用水蒸气蒸馏萃取法处理新鲜采摘的桂花，利用飞行时间质谱(GC-Q-TOF/MS)法定性分析β 紫罗兰酮，考察β 紫罗兰酮的碎裂方式，再用气相色谱三重四级杆串联质谱（GC-MS/MS）多重反应监测（MRM）定量分析桂花中β 紫罗兰酮。该方法在2.00 μg/L～100 μg/L范围内线性良好，方法检出限为8.67 ng/L。用该方法连续2 d测定新鲜桂花，β 紫罗兰酮均值为5.55 mg/kg。 |
| 英文摘要: |
| β ionone was extracted from fresh Osmanthus fragrans (Thunb.)Lour. by steam distillation, and was qualitatively analyzed by time of fight mass spectrometry(GC-Q-TOF/MS), quantitatively analyzed by gas chromatography-triple quadrupole tandem mass spectrometry(GC-MS/MS) through investigating its fragmentation manner. By optimizing the test conditions,the method had good linearity at 2.00 μg/L～100 μg/L of the target substance, the method detection limit was 8.67 ng/L. The method was applied in determining fresh Osmanthus fragrans (Thunb.)Lour. for 2 days, β-ionone was detected in two batches of samples, and the average test results was 5.55 mg/kg. |
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