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| UPLC-MS法同时测定蓝藻中BMAA和DAB |
| Simultaneous Determination of BMAA and DAB in Cyanobacteria by Ultra Performance Liquid Chromatography-Mass Spectrometry |
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| DOI： |
| 中文关键词: β-N-甲氨基-L-丙氨酸 2,4-二氨基丁酸 超高效液相色谱-质谱法 蓝藻 |
| 英文关键词: β-N-methylamino-L-alanine 2,4-diaminobutyric acid Ultra performance liquid chromatography-mass spectrometry Cyanobacteria |
| 基金项目:湖南省自然科学基金资助项目（2021JJ50135）；常德市科技创新专项基金资助项目（2020G178，2020S006） |
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| 中文摘要: |
| 用超高效液相色谱-质谱法（UPLC-MS）测定经6-氨基喹啉基-N-羟基琥珀酰亚胺基氨基甲酸酯（AQC）柱前衍生后的两种神经毒素β-N-甲氨基-L-丙氨酸（BMAA）和2,4-二氨基丁酸（DAB），通过优化试验条件，使方法在1.00 μg/L~250 μg/L范围内线性良好，BMAA 和DAB 的方法检出限分别为0.5 μg/L和0.9 μg/L。对空白蓝藻样品做低、中、高3个质量浓度水平的加标回收试验，BMAA和DAB的平均回收率分别为（88.2±4.0）%和（87.7±14.2）%，6次测定结果的RSD分别为2.6%~7.9%和2.1%~11.3%。 |
| 英文摘要: |
| Two neurotoxins,β-N-methylamino-L-alanine (BMAA)and 2,4-diaminobutyric acid(DAB) were determined by ultra-performance liquid chromatography-mass spectrometry(UPLC-MS), using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). By optimizing the test conditions, the method had good linearity in the range of 1.00 μg/L~250 μg/L. The detection limits of BMAA and DAB were 0.5 μg/L and 0.9 μg/L respectively. The average spiked recoveries of BMAA and DAB at low, medium and high concentration levels in blank samples were （88.2±4.0）% and （87.7±14.2）%, respectively, and the RSDs of 6 measurements were 2.6%~7.9% and 2.1%~11.3% respectively. |
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