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文章摘要
水中3种微囊藻毒素的高效液相色谱测定方法优化
Optimization of High-performance Liquid Chromatography Method for Determination of Three Microcystins in Water
  
DOI:
中文关键词: 微囊藻毒素  固相萃取  高效液相色谱法  水质
英文关键词: Microcystin  Solid phase extraction  HPLC  Water quality
基金项目:江西省水利厅科技课题“鄱阳湖饮用水源地微囊藻毒素时空分布研究”基金资助项目(201921YBKT22)
作者单位
沈友恒 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
刘发根 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
郭玉银 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
陈贤哲 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
张文彪 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
陈文滔 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
黄江 鄱阳湖水文水资源监测中心江西省鄱阳湖水文生态监测研究重点实验室 
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中文摘要:
      对天然水体中3种痕量微囊藻毒素(MC-RR、MC-YR、MC-LR)的淋洗、洗脱、液相色谱测定等环节进行单因素不同水平考察,结果表明:以体积分数为45%的甲醇水溶液(含0.1%的TFA)为固相萃取淋洗剂、10 mL甲醇(含01%TFA)为固相萃取洗脱剂,体积分数为70%的甲醇水溶液(含0.1%TFA)为液相色谱流动相,可在6 min内有效分离MC-RR、MC-YR、MC-LR。将优化后的方法用于实际加标水样的测定,MC-RR、MC-YR、MC-LR的方法检出限分别为0.064 μg/L、0.098 μg/L、0.095 μg/L,加标回收率为87.0%~116%,RSD为10.2%~18.8%。
英文摘要:
      The leaching, elution and liquid phase determination of three trace microcystins (MC-RR, MC-YR, MC-LR)in natural water were investigated at different levels by single factor. The results showed that MC-RR, MC-YR and MC-LR could be effectively separated within 6 minutes by using 45% methanol aqueous solution (containing 0.1% TFA) as solid phase extraction leacheate, 10 mL methanol (containing 0.1% TFA) as solid phase extraction eluent, and 70% methanol aqueous solution (containing 0.1% TFA) as liquid chromatographic mobile phase. The optimized method was applied to the determination of spiked recovery of actual samples. The method detection limits of MC-RR, MC-YR, MC-LR were 0.064 μg/L, 0.098 μg/L, 0.095 μg/L, respectively, the recoveries were 87.0%~116% and the RSDs were 10.2%~18.8%.
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