| The leaching, elution and liquid phase determination of three trace microcystins (MC-RR, MC-YR, MC-LR)in natural water were investigated at different levels by single factor. The results showed that MC-RR, MC-YR and MC-LR could be effectively separated within 6 minutes by using 45% methanol aqueous solution (containing 0.1% TFA) as solid phase extraction leacheate, 10 mL methanol (containing 0.1% TFA) as solid phase extraction eluent, and 70% methanol aqueous solution (containing 0.1% TFA) as liquid chromatographic mobile phase. The optimized method was applied to the determination of spiked recovery of actual samples. The method detection limits of MC-RR, MC-YR, MC-LR were 0.064 μg/L, 0.098 μg/L, 0.095 μg/L, respectively, the recoveries were 87.0%~116% and the RSDs were 10.2%~18.8%. |
