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| 极小短小杆菌强化狼尾草对Cd耐受性的研究 |
| Study on the Tolerance of Curtobacterium pusillum Enhanced Pennisetum alopecuroides to Cd |
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| DOI： |
| 中文关键词: 极小短小杆菌 狼尾草 镉 耐受性 植物修复 |
| 英文关键词: Curtobacterium pusillum Pennisetum alopecuroides Cadmium Tolerance Phytoremediation |
| 基金项目:国家自然科学基金资助项目（42072201）；安徽省高校协同创新基金资助项目（GXXT-2021-017）；铜陵市环保督察重点基金资助项目（232107） |
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| 摘要点击次数: 252 |
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| 中文摘要: |
| 以矿区原生植物狼尾草（Pennisetum alopecuroides）和极小短小杆菌（Curtobacterium pusillum）为研究对象，通过盆栽试验，在不同Cd污染水平土壤中接种极小短小杆菌，研究该菌种对狼尾草生长特性的影响，并考察狼尾草对土壤中Cd的耐受性。结果表明，接种极小短小杆菌能显著提高狼尾草的抗氧化酶活性，以及非酶抗氧化剂、渗透调节物质和金属硫蛋白（MTs）含量，可减轻Cd胁迫造成的氧化损害，改善狼尾草的生长。在600 mg/kg的Cd污染水平下，接种极小短小杆菌的狼尾草中过氧化物酶（POD）活性和谷胱甘肽（GSH）含量比未接种的对照组分别高16744%和8920%，可溶性糖（SS） 和MTs含量分别增加2781%和2532%，根长和株高显著提高5984%和4723%（P＜005）。 |
| 英文摘要: |
| In this study，C.pusillum and the native plant P.alopecuroides in the mining area were taken as the research objects. Through pot experiments， C.pusillum was inoculated in soils with different levels of Cd pollution to study its effects on the growth characteristics of P.alopecuroides and to investigate the tolerance of P.alopecuroides to Cd in soil. The results showed that inoculation with C.pusillum could significantly enhance the antioxidant enzyme activity of P.alopecuroides， increase the content of non enzymatic antioxidants， osmotic regulators and metallothionein（MTs） in P.alopecuroides， alleviate the oxidative damage caused by Cd stress and improve the growth of P.alopecuroides. When the Cd content was 600 mg/kg，the peroxidase（POD） activity and glutathione（GSH） content in P.alopecuroides inoculated with C.pusillum were 16744% and 8920% higher， respectively， than those in the uninoculated control group. The contents of soluble sugar（SS） and MTs increased by 2781% and 2532%， respectively. The root length and plant height were significantly increased by 5984% and 4723%（P<005），respectively. |
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