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| 苯系物好氧降解菌株ZX-1的分离鉴定和降解特性 |
| Isolation， Identification and Degradation Characteristics of BTEX Degrading Aerobic Bacterium ZX 1 |
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| DOI： |
| 中文关键词: 苯系物 红球菌属 微生物降解 分离鉴定 好氧环境 |
| 英文关键词: BTEX Rhodococcus sp. Microbial degradation Isolation and identification Aerobic environment |
| 基金项目:国家自然科学基金资助项目（42202281）；江苏省土壤与地下水污染防控工程研究中心自主课题基金资助项目（STRZX002）；江苏省卓越博士后计划基金资助项目（2023ZB141） |
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| 中文摘要: |
| 从某化工污染土壤中分离出一株苯系物好氧降解菌ZX-1，通过形态特征和16S rRNA基因序列分析方法鉴定菌株，利用控制变量法研究菌株降解特性，用顶空〖CD*2〗气相色谱法测定污染物。结果表明：菌株ZX-1为红球菌属（Rhodococcus），最适接菌量≥3%、温度为30 ℃、pH值为6~8，对青霉素、复方新诺明、氨苄西林、红霉素具有抗性；菌株ZX-1具有降解高浓度苯系物复合污染能力，60 h内对908 mg/L的苯、808 mg/L的甲苯和1057 mg/L的乙苯降解率＞900%，对842 mg/L的邻〖CD*2〗二甲苯和1074 mg/L的对/间〖CD*2〗二甲苯降解率＞750%。 |
| 英文摘要: |
| In this study， a BTEX degrading aerobic bacterium ZX 1 was isolated from chemically contaminated soil，and identified by morphological characteristics and 16S rRNA gene sequence analysis. The degradation characteristics of the strain were studied by control variable method， and the pollutants were monitored by headspace gas chromatography. The results showed that strain ZX 1 belonged to genus Rhodococcus. The optimal inoculation amount was ≥3%， with a temperature of 30 ℃ and pH range of 6~8. The strain was resistant to penicillin， trimethoprim sulfamethoxazole，ampicillin and erythromycin， and had the ability to degrade high concentration BTEX. 〖JP3〗Within 60 h， the degradation rates for 908 mg/L benzene， 808 mg/L〖JP〗 toluene and 1057 mg/L ethylbenzene exceeded 900%， 〖JP3〗while the degradation rates for 842 mg/L o xylene and 1074 mg/L〖JP〗 p/m xylene exceeded 750%. |
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