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| eDNA采样时空优化对长江上游长寿段鱼类多样性监测结果的影响 |
| Effects of Spatiotemporal Optimization of eDNA Sampling on Fish Diversity Monitoring in the Changshou Section of the Upper Yangtze River |
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| DOI： |
| 中文关键词: 鱼类多样性 环境DNA技术 昼夜节律 采样方案 长江上游 长寿段 |
| 英文关键词: Fish diversity Environmental DNA technology Diurnal rhythm Sampling design Upper reaches of the Yangtze River Changshou section |
| 基金项目:国家重点研发计划基金资助项目（2024YFC3212500） |
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| 摘要点击次数: 154 |
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| 中文摘要: |
| 通过设计涵盖采样点空间布置、昼夜周期与采样频次的多维采样方案，系统评估其对环境DNA（eDNA）宏条形码技术测定河流鱼类多样性结果的影响。试验表明：长江上游长寿段共检出鱼类111种，包括11种国家重点保护鱼类和30种长江特有鱼类；采样点位空间分布及采样时间的昼夜跨度对鱼类物种检出结果具有显著影响，夜间采样使鲿科、鲇科等夜间活动鱼类检出率显著提升；跨昼夜连续12次采样可实现近90%的物种累计检出率。可根据栖息地特征优化空间布点，结合昼夜节律适当增加样本量，以提升大型河流eDNA监测的准确性。 |
| 英文摘要: |
| A multidimensional sampling design integrating spatial arrangement of sampling sites， diel variation， and sampling frequency was developed to systematically evaluate their effects on riverine fish diversity assessment using environmental DNA（eDNA） metabarcoding. Tests showed that， in the Changshou section of the upper Yangtze River，a total of 111 fish species were detected， including 11 nationally protected species and 30 species endemics to the Yangtze River. Species detection was significantly influenced by both the spatial distribution of sampling sites and the diel timing of sampling. Notably， nighttime sampling markedly improved the detection of nocturnal taxa， particularly members of the families Bagridae and Siluridae. Moreover， 12 consecutive sampling events spanning the diel cycle achieved nearly 90% 〖JP2〗of the cumulative species richness. Sampling sites can be optimized according to habitat characteristics， and sample size can be appropriately increased in consideration of circadian rhythms， so as to improve the accuracy of eDNA monitoring in large rivers. |
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